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KMID : 0350519950480020597
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Journal of Catholic Medical College
1995 Volume.48 No. 2 p.597 ~ p.611
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Effects of Fluoride on Mouse Fibroblast(L929)
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Abstract
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Titanium has good biocompatibility and high resistance to corrosion but may be corroded in the presence of fluorides widely used to prevent dental caries. Fluoride may be very toxic and can affect the attachment of cells to titanium surface.
This study was designed to observe the effect of sodium fluoride (NaF) on the attachment of mouse fibroblast (L929) to titanium surface and to evaluate cytotoxicity of NaF and to understand the mechanisms.
After L929 cells were incubated on the titanium surface in MEM containing 0.0015 and 0.015% NaF for 24 hours, the attachment of cells to titanium was observed by scanning electron microscope. In addition the cell viability against 0.25%-2% NaF
was
evaluated by neutral red assay method for 0.5-24 hours and the morphologic changes of cells were examined with time by transmission electron microscope.
@ES The results were as follows ;
@EN 1. On SEM examination, the cells on titanium specimen were less attached and spreaded than those on culture dish.
2. The cytotoxicity of NaF was significantly increased with the concentrations and incubation time (p<0.05).
3. There were a number of ultrastructural changes in NaF treatment group. The discontinuity of cell membrane and blebbing were observed. Endoplasmic reticulum and mitochondria were dilated and polyribosomes were dispersed and decreased in
number.
These findings suggest that the use of high concentration of fluoride should be avoided to patients with titanium implant and controlled cautiously in any occasion due to high toxic effects.
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